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10.1016/j.mce.2016.04.024 223 ShehzadA.LeeY

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Although they initially originated from the parent variant selected for the fastest growth on glucose-ammonia minimal medium, auxotrophs required threonine (strain 23), nicotinic acid (strain 122), or tryptophan (strains 160, 166, and 168) due to damage of some key metabolic genes

doi: 10.4103/jhrs.JHRS_106_18

Ejaculates from other species have shown similar but not identical banding characteristics: small changes to the precise densities of the layers may be required