how does glp-1 affect the brain in Hypothalamic Paraventricular Nucleus Promotes Sympathetic Activation and Hypertension Mechanisms of GLP-1 in Modulating

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Using a 1:100 ratio of mg tissue to L KME buffer (20 mM MOPS, 120 mM KCl, and 1 mM EGTA, pH 7.4), tissue was homogenized in 2 s bursts separated by a 2 s rest for a total of 10 s (or 3 cycles) at 16,600 rpm using IKA T25 digital Ultra-Turrax homogenizer

Delgado CA, Balbueno Guerreiro GB, Diaz Jacques CE, de Moura Coelho D, Siita A, Manfredini V, Wajner M, Vargas CR (2019) Prevention by L-carnitine of DNA damage induced by 3-hydroxy-3-methylglutaric and 3-methylglutaric acids and experimental evidence of lipid and DNA damage in patients with 3-hydroxy-3-methylglutaric aciduria