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A test is useful to confirm deficiency, but its not always required before starting a private B12 injection course

According to a 2019 study, the rate at which vitamins lose their potency depends on the form of the supplement, the storage methods, and the date of manufacture

B12 therapy may also support healthy immune function, and some studies have even suggested that it may reduce the risk of certain types of cancer

doi:10.1038/s42255-023-00916-6

There is no direct trek between the two (like the one from Choquequirao to Vilcabamba, which is very advanced), but many do it as part of a longer trip through southern Peru