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Cells were lysed at 4 C in a radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Biotechnology)

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Second, as compared with AHS animals, the circulating levels of chemokines (i.e., SDF-1 and GLP-1) and EPCs (i.e., C-kit/CD31+, Sca-1/CD31+, KDR/CD34+ and VE-Cadherin/CD34+) were remarkably increased in DPP4 D and Sita-treated animals, suggesting these results were mainly due to the suppression of DPP4 enzyme activity