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We therefore measured G protein-dependent cAMP generation and -arrestin-1 and -2 recruitment with exendin-4, exendin-phe1, and exendin-asp3 at two time-points to avoid kinetic artefacts 38 , and calculated the pathway bias by fitting the response data to a modified form of the operational model of agonism 39 (Fig
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10,14 The light, modifier subunit provides a regulatory function, while GCLC is responsible for the catalytic activity, which conjugates sulfur-containing cysteine with glutamate, forming -glutamylcysteine
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