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The PCR products were digested with Nco I and Bam HI and ligated into the pKM260 expression vector (EUROSCARF, Frankfurt, Germany), which resulted in the N-terminal addition of a hexahistidine tag and linker with the sequence MHHHHHHASENLYFQGAM

Patients with PD had thinner average RNFLs, indicating potential diagnostic significance of RNFL thickness (Alves et al., 2023) (see Table 7)

It seems that weight loss will likely be very important for the benefits of the GLP-1 medicines in some conditions, and much less important, or not at all essential, for benefits in other disorders. According to Lane, although GLP-1 receptor agonists show promise in reducing cardiovascular risk factors, long-term cardiovascular safety trials are needed to assess cardiovascular mortality and events